Question

Which of these would not be used to introduce DNA in animal cells?

• A. Liposomes
• B. Electroporation
• C. Microinjection
• D. Ti-plasmid

Hint:

While Ti-plasmid is key in plant genetic engineering, other techniques such as liposomes, electroporation, and microinjection are common methods used for DNA introduction into animal cells.

Answer 1

The Correct Option is D

Ti-plasmid is a plasmid used by Agrobacterium to transfer DNA into plant cells, not animal cells. The Ti-plasmid is widely used in plant genetic engineering but not in animal cell transformation.

(1) Liposomes (Option 1) can be used to introduce DNA into animal cells. Liposomes are lipid-based vesicles that can fuse with the cell membrane, facilitating the entry of DNA into the cell.

(2) Electroporation (Option 2) is another method used to introduce DNA into animal cells by applying an electric field to increase cell membrane permeability.

(3) Microinjection (Option 3) is a technique where DNA is directly injected into animal cells, typically used for precise DNA delivery.

Conclusion: The Ti-plasmid is not used to introduce DNA into animal cells, as it is specific to plant cell transformation.