Question

Which of the following techniques uses temperature cycling for DNA amplification?

• A. DNA fingerprinting
• B. PCR
• C. RAPD
• D. Northern blotting

Hint:

In PCR, temperature cycling is a key step that enables DNA amplification through denaturation, annealing, and extension.

Answer 1

The Correct Option is B

Polymerase Chain Reaction (PCR) uses repeated cycles of heating and cooling to amplify specific DNA sequences. This temperature cycling is essential for denaturing the DNA, annealing primers, and extending the DNA strands.