Question

Which of the following techniques is commonly used to identify protein-protein interaction?

• A. Southern blotting
• B. Yeast two-hybrid
• C. Northern blotting
• D. Western blotting

Hint:

Remember: - Southern = DNA
- Northern = RNA
- Western = Protein
- Yeast two-hybrid = Protein-protein interactions

Answer 1

The Correct Option is B

Step 1: Understand the techniques mentioned
Southern blotting: used for detecting specific DNA sequences.
Northern blotting: used for detecting specific RNA sequences.
Western blotting: used for detecting specific proteins.
Step 2: Identify the method for protein-protein interaction
The yeast two-hybrid system is a molecular biology technique used specifically to study protein-protein interactions. It uses a split transcription factor that activates a reporter gene only when two proteins of interest physically interact.