Question

Which of the following methods are used for detection of DNA and RNA, respectively?

• A. Southern and Northern blotting
• B. Southern and Western blotting
• C. Northern and Southern blotting
• D. Northern and Western blotting

Hint:

Southern → DNA
Northern → RNA
Western → Proteins
Eastern → Post-translational modifications

Answer 1

The Correct Option is A

Step 1: Southern blotting is a molecular biology technique used specifically for detection of DNA sequences.
Step 2: Northern blotting is the analogous method used for detection of RNA sequences.
Step 3: Western blotting is used for proteins, not nucleic acids.
Step 4: Hence, for DNA and RNA detection respectively, the answer is Southern and Northern blotting.