Question

Which of the following is the disadvantage of reciprocating pump used in liquid chromatography?

• A. Produces pulsed flow
• B. Corrosive components
• C. Does not have small hold-up value
• D. Does not have moderate flow rate

Answer 1

The Correct Option is A

Step 1: Understanding reciprocating pumps in liquid chromatography. - Reciprocating pumps are commonly used in High-Performance Liquid Chromatography (HPLC) due to their high-pressure capabilities and small hold-up volume.

Step 2: Identifying the disadvantage of reciprocating pumps. - Pulsed Flow: Reciprocating pumps operate by moving pistons back and forth, leading to flow pulsations. - Flow pulsation can cause fluctuations in chromatography, affecting retention times and reproducibility.

Step 3: Explanation of incorrect options. - (B) Corrosive components: Incorrect, as reciprocating pumps are designed to handle various solvents, including corrosive ones. - (C) Does not have small hold-up value: Incorrect, because reciprocating pumps have a small internal volume, ensuring minimal hold-up. - (D) Does not have moderate flow rate: Incorrect, as reciprocating pumps allow precise control over flow rate.

Step 4: Selecting the correct option. Since the major drawback of reciprocating pumps is the production of pulsed flow, the correct answer is (A) Produces pulsed flow.