Question

Which amino acid gives max UV absorption?

• A. \( \text{Tryptophan} \)
• B. \( \text{Tyrosine} \)
• C. \( \text{Phenylalanine} \)
• D. \( \text{Proline} \)

Hint:

UV spectroscopy is commonly used to analyze protein concentrations by measuring absorption at 280 nm.

Answer 1

The Correct Option is A

Tryptophan exhibits the highest UV absorption due to its aromatic indole ring. Its absorbance peak is around 280 nm, making it useful for protein quantification in spectrophotometric analysis. Other aromatic amino acids, such as tyrosine and phenylalanine, also absorb UV light but to a lesser extent.