The determinant of a 2×2 matrix
\[
\begin{pmatrix} a & b \\ c & d \end{pmatrix}
\]
is given by:
\[
\text{Determinant} = ad - bc.
\]
Substituting the values:
\[
\text{Determinant} = (-2)(1) - (5)(-4) = -2 + 20 = 16.
\]
Hence, the correct answer is A) 16.