Blood serum: Blood serum, specifically fetal bovine serum (FBS), is a rich source of peptide hormones, growth factors and other nutrients which are necessary for the healthy in vitro growth of animal cells. These components provide the cells with the necessary signals for proliferation and differentiation.
Phenol red: Phenol red is a pH indicator used in cell culture media to monitor the pH levels.
Antibiotics: Antibiotics are added to cell culture media to prevent bacterial contamination.
Amino acids: Amino acids are components of cell culture media but do not serve the purpose of providing peptide hormones and growth factors.