Question

The introduction of nematode-specific genes into host using Agrobacterium vectors causes the following action:
I. Formation of dsRNA.
II. Production of sense and antisense RNAs in the host cells.
III. RNA intermediate not formed.
IV. Silencing of specific mRNA of the nematode.

• A. II, III, IV
• B. I, II, III
• C. I, III, IV
• D. I, II, IV

Hint:

RNAi in plants involves sense and antisense RNA leading to silencing of target mRNA.

Answer 1

The Correct Option is A

In gene silencing technology, the host plant produces both sense and antisense RNA strands which form dsRNA. This dsRNA does not proceed through a normal RNA intermediate and causes silencing of specific nematode genes through RNA interference. Hence II, III, and IV are valid consequences.