Question:

The cloning site present in the tetracycline resistance gene of \textit{E. coli cloning vector pBR322 is:

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Sal I is used to insert DNA into the tetracycline resistance gene of pBR322.
Updated On: Jun 19, 2025
  • EcoR I
  • Pvu II
  • Sal I
  • Pst I
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The Correct Option is C

Solution and Explanation

In pBR322, the Sal I restriction site is present in the tetracycline resistance gene. Insertion at this site disrupts the tetracycline resistance, allowing for identification of recombinants.
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