The cloning site present in the tetracycline resistance gene of \textit{E. coli cloning vector pBR322 is:
Show Hint
- EcoR I
- Pvu II
- Sal I
- Pst I
The Correct Option is C
Solution and Explanation
Top Questions on Applications of Biotechnology
- Explain why the milk produced by the mother during the initial days of lactation is considered to be very essential for the newborn infant.
- CBSE CLASS XII - 2025
- Biology
- Applications of Biotechnology
Answer the following questions:
(a) [(i)] Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
[(ii)] How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists?
(b) [(i)] Explain how the amplification of gene of interest is done using PCR.
[(ii)] State two applications of the desired amplified fragment of DNA.
- CBSE CLASS XII - 2025
- Biology
- Applications of Biotechnology
- Explain the beneficial role of the following, produced as a result of the processes of biotechnology, to mankind:
(a) Cow named Rosie
(b) \(\alpha\)-1-antitrypsin- CBSE CLASS XII - 2025
- Biology
- Applications of Biotechnology
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a) What is the technical term used for Step 4 in the above process?
(b) Which of the given two combinations of restriction enzyme should be used in Step 1? Justify your answer.
(i) EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii) EcoR I to cut both the plasmid and alien DNA.- CBSE CLASS XII - 2025
- Biology
- Applications of Biotechnology
- Assertion (A): Isolated single cells can be fused to produce somatic hybrids.
Reason (R): Cells selected for somatic hybridisation have desirable characters.- CBSE CLASS XII - 2025
- Biology
- Applications of Biotechnology
Questions Asked in CBSE CLASS XII exam
- “Where there is a will, there is a way.” The above saying aptly describes the character of Douglas from ‘Deep Water’ and Mukesh from ‘Lost Spring’. Compare and contrast their characters in terms of their courage, determination, and the pursuit of their goals.
- CBSE CLASS XII - 2025
- Literature
- If \( A \) denotes the set of continuous functions and \( B \) denotes the set of differentiable functions, then which of the following depicts the correct relation between set \( A \) and \( B \)?
- CBSE CLASS XII - 2025
- Set Theory and types of Sets
In the given reaction sequence, the structure of Y would be:
- CBSE CLASS XII - 2025
- Aldehydes, Ketones and Carboxylic Acids
- Prove that, in the Bohr model of the hydrogen atom, the time period of revolution of an electron in the \( n \)-th orbit is proportional to \( n^3 \).
Sudha and Sudhir were partners in a firm sharing profits and losses in the ratio of 4 : 1. On 1st April, 2023, their fixed capitals were ₹12,00,000 and ₹4,00,000 respectively. On 1st July, 2023, Sudha invested ₹2,00,000 as additional capital. On 1st August, 2023, Sudhir withdrew ₹50,000 from his capital.
The partnership deed provided for the following:
(i) Interest on capital @ 6% p.a.
(ii) Interest on drawings @ 8% p.a.
During the year, Sudha withdrew ₹60,000 and Sudhir withdrew ₹40,000 for personal use. After providing interest on capital and charging interest on drawings, the net profit of the firm for the year ended 31st March, 2024 was ₹3,50,000.
Prepare Current Accounts of Sudha and Sudhir.- CBSE CLASS XII - 2025
- Partnership Accounts