Question:

Rop-gene which codes for the proteins involved in the replication of the plasmid pBR322 in E.coli is located at restriction site of

Updated On: Apr 1, 2025
  • Hind III
  • EcoRI
  • Pvu II
  • BamH I
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The Correct Option is C

Solution and Explanation

The rop gene in plasmid pBR322 codes for proteins involved in the replication of the plasmid. The rop gene is located near the PvuII restriction site in the pBR322 plasmid.

  • HindIII, EcoRI, BamHI: These are other restriction enzymes that cut at specific sites in DNA but are not the location of the rop gene.
  • PvuII: This is the correct restriction site where the rop gene is located.

The correct answer is (C) : Pvu II.

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