Question:

Principle of paper chromatography is based on

Updated On: Jun 14, 2022
  • solid-liquid partition chromatography
  • liquid-partition chromatography
  • liquid-solid adsorption chromatography
  • liquid-liquid sorption chromatography
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The Correct Option is B

Solution and Explanation

Principle of paper chromatography is based on liquid - liquid partition chromatography. In paper chromatography, special quality paper known as chromatography paper contains water trapped in it which acts as the stationary phase. A strip of chromatographic paper spotted at the base with the solution of the mixture is suspended in a suitable solvent for a mixture of solvents. This solvent acts as the mobile phase. The solvent rises up the paper by capillary action and flows over the spot. The paper selectively retains different components according to their differing partition in the two phases. The paper strip so developed is called as a chromatogram.
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Concepts Used:

Adsorption

Heinrich Kayser, the German physicist was the first to coin the term adsorption. Adsorption can be explained as a surface phenomenon where particles remain attached on the top of a material. Generally, it comprises the molecules, atoms, liquid, solid in a dissolved stage, even the ions of a gas that are attached to the surface. Much to our surprise, the consequence of surface energy i.e. adsorption is present in biological, physical, chemical, and natural systems and are used in many industrial applications.