Question

Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?

• A. They add A residue at 3' end
• B. They are obtained from Thermococcus litoralis
• C. They can't be obtained from archaebacteria
• D. The marine bacteria from which they are obtained grow at temperatures lower than that of Thermus aquatics

Hint:

For high-fidelity PCR, choose polymerases that have proofreading activity, like those from Thermococcus litoralis.

Answer 1

The Correct Option is B

Polymerases with proofreading activity, such as Taq polymerase, can be obtained from thermophilic bacteria like Thermococcus litoralis. These polymerases have exonuclease activity, allowing them to correct errors during DNA replication. This proofreading capability is a valuable characteristic for high-fidelity PCR amplification.