Question

Only what percentage of the effluent of liquid chromatography must be introduced into the mass spectrometer?

• A. 1–20%
• B. 1–15%
• C. 1–5%
• D. Below 1%

Hint:

Proper LC-MS tuning ensures optimal ionization efficiency and accurate mass spectral interpretation.

Answer 1

The Correct Option is C

In Liquid Chromatography-Mass Spectrometry (LC-MS), typically 1-5% of the total effluent is directed into the mass spectrometer. This ensures efficient ionization while avoiding overloading and contamination of the MS instrument. Excess sample introduction can reduce detection sensitivity and affect mass spectral accuracy.