Question:
Name the process involved in the production of nematode-resistant tobacco plants, using genetic engineering. Explain the strategy adopted to develop such plants.
Name the process involved in the production of nematode-resistant tobacco plants, using genetic engineering. Explain the strategy adopted to develop such plants.
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RNA interference (RNAi) uses dsRNA to silence specific genes — a powerful tool in developing pest- or pathogen-resistant crops.
Updated On: Jun 20, 2025
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Solution and Explanation
Process Name: RNA interference (RNAi) Strategy Adopted:
- RNA interference is a gene-silencing method that involves the introduction of double-stranded RNA (dsRNA) that is complementary to the mRNA of the target gene.
- In the case of nematode-resistant tobacco, a gene coding for a nematode protein is inserted into the plant in such a way that it produces both sense and antisense RNA strands.
- These strands form dsRNA, which triggers the RNAi mechanism, degrading the nematode's mRNA when it infects the plant.
- As a result, the nematode fails to survive due to the lack of synthesis of vital proteins.
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