Question:

What is the primary purpose of using T4 DNA ligase in recombinant DNA technology?

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T4 DNA ligase is essential for sealing DNA fragments together, a key step in cloning and plasmid construction.

Updated On: Sep 20, 2025

To cut DNA at specific sequences
To join DNA fragments with complementary sticky ends
To synthesize DNA from RNA
To unwind DNA for replication

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The Correct Option is B

Solution and Explanation

Step 1: Understand the role of T4 DNA ligase.
T4 DNA ligase is used to catalyze the formation of phosphodiester bonds between adjacent nucleotides, effectively joining DNA fragments with complementary sticky ends. This is a crucial step in recombinant DNA technology.

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Questions Asked in CUET PG exam

Match the LIST-I with LIST-II
LIST-I (Energy of a particle in a box of length L) LIST-II (Degeneracy of the states)
A. \( \frac{14h^2}{8mL^2} \) I. 1
B. \( \frac{11h^2}{8mL^2} \) II. 3
C. \( \frac{3h^2}{8mL^2} \) III. 6

Choose the correct answer from the options given below:
- CUET (PG) - 2025
- Quantum Mechanics
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LIST-I (Energy of a particle in a box of length L)	LIST-II (Degeneracy of the states)
A.	\( \frac{14h^2}{8mL^2} \)	I.	1
B.	\( \frac{11h^2}{8mL^2} \)	II.	3
C.	\( \frac{3h^2}{8mL^2} \)	III.	6

Match the LIST-I with LIST-II

LIST-I (Type of decay in Radioactivity)		LIST-II (Reason for stability)
A.	Alpha decay	III.	Nucleus is mostly heavier than Pb (Z=82)
B.	Beta negative decay	IV.	Nucleus has too many neutrons relative to the number of protons
C.	Gamma decay	I.	Nucleus has excess energy in an excited state
D.	Positron Emission	II.	Nucleus has too many protons relative to the number of neutrons