Question

What is the primary purpose of using T4 DNA ligase in recombinant DNA technology?

• A. To cut DNA at specific sequences
• B. To join DNA fragments with complementary sticky ends
• C. To synthesize DNA from RNA
• D. To unwind DNA for replication

Hint:

T4 DNA ligase is essential for sealing DNA fragments together, a key step in cloning and plasmid construction.

Answer 1

The Correct Option is B

Step 1: Understand the role of T4 DNA ligase.
T4 DNA ligase is used to catalyze the formation of phosphodiester bonds between adjacent nucleotides, effectively joining DNA fragments with complementary sticky ends. This is a crucial step in recombinant DNA technology.