Question:
Methods for inserting recombinant DNA into host:
I. Selectable marker
II. Disarmed pathogen vector
III. Biolistic method
IV. Microinjection
Methods for inserting recombinant DNA into host:
I. Selectable marker
II. Disarmed pathogen vector
III. Biolistic method
IV. Microinjection
I. Selectable marker
II. Disarmed pathogen vector
III. Biolistic method
IV. Microinjection
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Only II, III, IV are physical/biological DNA transfer methods; selectable marker is for screening.
Updated On: Jun 2, 2025
- I, II, III
- II, III, IV
- I, III, IV
- I, II, IV
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The Correct Option is B
Solution and Explanation
- Selectable marker is not a method of transfer, it's used for selecting transformed cells.
- Disarmed pathogen vector — method using modified Agrobacterium plasmids.
- Biolistic method — gene gun, physical method.
- Microinjection — DNA injected directly into nucleus.
- Disarmed pathogen vector — method using modified Agrobacterium plasmids.
- Biolistic method — gene gun, physical method.
- Microinjection — DNA injected directly into nucleus.
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