Question

Mention the Nitrogen base sequence name, restriction cut sites and type of cut ends in the above DNA fragment.
5' - GAATTC - 3'
3' - CTTAAG - 5'

• A. Recognition sequence, A & A, cohesive ends
• B. Palindrome sequence, G & A, sticky ends
• C. Endonuclease, T & T, staggered cut
• D. Ligase sites, C & T, sticky ends

Hint:

EcoRI → GAATTC → sticky ends; palindrome = same forward/reverse complement.

Answer 1

The Correct Option is B

The sequence GAATTC is recognized by the restriction enzyme EcoRI and is a palindromic sequence. It cuts between G and A, resulting in sticky ends which are single-stranded overhangs.