List-I Products | List-II Organisms |
---|---|
(A) Statin | (III) Monascus |
(B) Clot buster | (I) Streptococcus |
(C) Swiss cheese | (IV) Propionibacterium |
(D) Cyclosporin-A | (II) Trichoderma |
The task is to match products listed in List-I with the corresponding organisms in List-II.
List-I Products | List-II Organisms |
---|---|
(A) Statin | (III) Monascus |
(B) Clot buster | (I) Streptococcus |
(C) Swiss cheese | (IV) Propionibacterium |
(D) Cyclosporin-A | (II) Trichoderma |
Explanation for the correct matches:
The correct matches according to the given lists are: (A) - (III), (B) - (I), (C) - (IV), (D) - (II).
This question lists several industrially important microbial products and the microorganisms from which they are derived.
Explanation: Statins are a class of drugs used to lower cholesterol levels in the blood. Monascus purpureus is a type of red yeast that naturally produces statins.
Explanation: Streptokinase is an enzyme that can dissolve blood clots and is used to treat conditions such as heart attacks and pulmonary embolism.
Explanation: Propionibacterium freudenreichii ferments lactic acid, producing propionic acid, acetic acid, and carbon dioxide. The carbon dioxide forms the characteristic "eyes" in Swiss cheese, and the other products contribute to its flavor.
Explanation: Cyclosporin A is used to suppress the immune system, especially in transplant patients to prevent organ rejection.
Answer the following questions:
(a) [(i)] Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
[(ii)] How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists?
(b) [(i)] Explain how the amplification of gene of interest is done using PCR.
[(ii)] State two applications of the desired amplified fragment of DNA.
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a) What is the technical term used for Step 4 in the above process?
(b) Which of the given two combinations of restriction enzyme should be used in Step 1? Justify your answer.
(i) EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii) EcoR I to cut both the plasmid and alien DNA.