Question:

In the extraction of genetic material (DNA), which enzyme is not used?

Updated On: Aug 1, 2023
  • Ribonuclease
  • Cellulose
  • Protease
  • Both Protease and Ribonuclease
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The Correct Option is B

Solution and Explanation

The correct answer is option (B): Cellulose
Ribonuclease is used during DNA extraction to remove the contamination of RNA. Ribonuclease helps in the cleavage of RNA into smaller fragments thus confirming the purity of DNA. Protease on the other hand is used to remove protein contamination during DNA extraction. Protease cleaves the peptide bond in proteins. Cellulose is not directly involved during DNA extraction. 
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Concepts Used:

Applications of Biotechnology

Biotechnology is the technology that utilizes biological systems, living organisms, or parts of this to develop or create different products.

For example, brewing and baking bread fall within the concept of biotechnology (use of yeast (= living organism) to produce the desired product).

Agriculture

Alternative to conventional farming: A possible solution is the use of genetically modified crops which is an alternative path to conventional farming.

Medicine 

In the area of healthcare, recombinant DNA technological processes have made an immense impact which enables the mass production of safe and more effective therapeutic drugs.

Gene Therapy 

A collection of methods that allows the correction of a gene defect that has been diagnosed in a child/embryo is called Gene Therapy.

CRISPR technology

For editing genomes, CRISPR is a simple yet powerful tool which allows researchers to easily alter DNA sequences and modify gene function.