Question

In reverse phase chromatography, the stationary phase is made by

• A. Non-polar
• B. Polar
• C. Both (a) and (b)
• D. None of these

Answer 1

The Correct Option is A

Step 1: Understanding reverse phase chromatography - Reverse phase chromatography is a liquid chromatography technique where the stationary phase is non-polar and the mobile phase is polar. - It is commonly used for separating non-polar compounds in complex mixtures.

Step 2: Explanation of stationary phase - In reverse phase chromatography, the stationary phase is hydrophobic (non-polar), typically made of silica bonded with hydrocarbons like C8 or C18 chains. - The mobile phase is polar, typically a mixture of water, methanol, or acetonitrile.

Step 3: Evaluating the options - (A) Non-polar → Correct, as reverse phase chromatography uses a non-polar stationary phase. - (B) Polar → Incorrect, as polar stationary phases are used in normal-phase chromatography. - (C) Both (a) and (b) → Incorrect, as the stationary phase is strictly non-polar. - (D) None of these → Incorrect, as option (A) is correct.

Step 4: Selecting the correct answer Since reverse phase chromatography uses a non-polar stationary phase, the correct answer is (A) Non-polar.