Question

Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in the construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near the anode while larger fragments are found near the wells in an agarose gel.

• A. Both statement I and statement II are incorrect.
• B. Statement I is correct but statement II is incorrect.
• C. Statement I is incorrect but statement II is correct.
• D. Both statement I and statement II are correct.

Hint:

Gel electrophoresis: Small fragments travel farther. Recombinant DNA uses these fragments.

Answer 1

The Correct Option is D

Both statements are correct. DNA fragments separated by gel electrophoresis can be extracted and used for various molecular biology applications, including constructing recombinant DNA molecules. In gel electrophoresis, DNA migrates towards the positive electrode (anode) because DNA is negatively charged. Smaller fragments move faster and farther than larger fragments, which remain closer to the wells where the DNA was initially loaded.