Eukaryotic genes are monocistronic but they are split genes because
The correct answer is: (D) Exons are interrupted by Introns.
Eukaryotic genes are described as monocistronic, meaning each gene encodes a single protein. However, they are also called "split genes" because the coding sequence of the gene is interrupted by non-coding regions called introns.
Exons are the regions of the gene that contain the actual coding information for the protein. These exons are separated by introns, which do not code for any protein. During the process of transcription, the entire gene, including both exons and introns, is transcribed into mRNA. However, before the mRNA is translated into a protein, the introns are spliced out, leaving only the exons to form the mature mRNA.
This "splitting" of coding regions by non-coding introns is a defining characteristic of eukaryotic genes, differentiating them from prokaryotic genes, which are typically colinear (uninterrupted) between the gene and the protein it codes for.
Thus, the correct explanation for eukaryotic genes being "split" is that Exons are interrupted by Introns.
Arrange the following in correct order in polymerase chain reaction cycle:
I Deannealing
II Denaturation
III Extension
IV Annealing