Question

________________________ cultures can be maintained for a prolonged period of time by repeated sub-culturing.

• A. Ovary
• B. Protoplast
• C. Callus
• D. Mass cell

Hint:

Make sure to note that plant tissue culture uses callus for long term experiments, and other structures like protoplast for short term experiments.

Answer 1

The Correct Option is C

Callus Cultures: Callus is an undifferentiated mass of cells that are commonly used in plant tissue culture. They can be maintained in the lab for long periods through repeated sub-culturing. They are capable of growing for indefinite periods and can be used for various tissue engineering processes. Ovary: Ovaries are parts of the plant reproductive system which contain ovules and are not typically used in long term cultures in vitro. Protoplast: Protoplasts are plant cells from which the cell wall has been removed. Protoplasts are useful in plant genetic engineering and transformation techniques, and they are used in short term experiments. Mass Cell: The term ‘Mass Cell’ is not associated with plant cell culture.