Question

Cryopreservation is done at a temperature of:

• A. \(-140\,^\circ\text{C}\)
• B. \(-120\,^\circ\text{C}\)
• C. \(-196\,^\circ\text{C}\)
• D. \(-273\,^\circ\text{C}\)

Hint:

Cryopreservation uses liquid nitrogen at \(-196\,^\circ\text{C}\) to halt biological activity and preserve samples.

Answer 1

The Correct Option is C

Cryopreservation is a technique used to preserve cells, tissues, or any biological constructs by cooling them to extremely low temperatures. The commonly used temperature for this process is: \[ -196\,^\circ\text{C} \] This is the boiling point of liquid nitrogen, which is widely used as the cryogenic medium for storage.
At this temperature, all metabolic and biochemical processes are effectively halted, thereby preserving the biological sample without damage for extended periods.
- \(-273\,^\circ\text{C}\) is absolute zero — not practically achievable.
- \(-120\,^\circ\text{C}\) and \(-140\,^\circ\text{C}\) are cold but not standard for long-term cryopreservation.

Answer 2

Cryopreservation is done at a temperature of:

Cryopreservation is a technique used to preserve cells, tissues, or any biological constructs by cooling them to very low temperatures. This process halts all biological activity, including biochemical reactions that might cause cell damage or death.

The temperature commonly used for cryopreservation is −196°C, which is the boiling point of liquid nitrogen. At this temperature, biological materials can be stored for long periods without degradation because molecular motion virtually stops, preventing ice crystal formation that could otherwise damage cellular structures.

Thus, liquid nitrogen at −196°C is the standard medium for cryopreservation in laboratories and medical facilities.

Correct Answer: −196°C