Question

Choose the components that are required for the proper functioning of DNA polymerase enzyme.
(P) DNA template
(Q) A DNA primer
(R) An RNA primer
(S) Four different dNTPs

• A. Only (P), (Q) and (R)
• B. Only (P), (R) and (S)
• C. Only (Q), (R) and (S)
• D. Only (P), (Q) and (S)

Hint:

In PCR (in-vitro), we use DNA primers, but in the biological context of the enzyme's proper functioning in a cell, RNA primers are the standard requirement.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
DNA polymerase is the enzyme that synthesizes a new DNA strand. It has specific requirements for initiation and elongation.

Step 2: Detailed Explanation:
1. DNA Template (P): Required to guide the sequence of the new strand.
2. Primer: DNA polymerase cannot start a new strand from scratch. In natural replication (in-vivo), it uses an RNA primer (R).
3. dNTPs (S): These are the building blocks (dATP, dTTP, dGTP, dCTP) and also provide energy.

Step 3: Final Answer:
Components (P), (R), and (S) are required. Option (b) is correct.