Question:
Blue-white screening is performed to select .......... in vector-based molecular cloning.
Blue-white screening is performed to select .......... in vector-based molecular cloning.
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Blue-white screening is a valuable tool in molecular cloning to identify successful transformations by detecting recombinant bacteria.
Updated On: May 6, 2025
- Vectors from plasmid
- Gram-positive bacteria from Gram-negative bacteria
- Antibiotic-resistant bacteria
- Recombinant bacteria from non-recombinant bacteria
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The Correct Option is D
Solution and Explanation
Let's analyze each option:
(1) Vectors from plasmid: Blue-white screening is used to select recombinant bacteria, not vectors from plasmids. Hence, this is not the correct answer.
(2) Gram-positive bacteria from Gram-negative bacteria: Blue-white screening does not differentiate between Gram-positive and Gram-negative bacteria. This is not the correct answer.
(3) Antibiotic-resistant bacteria: While antibiotic resistance may be involved in cloning, blue-white screening is specifically used to distinguish recombinant bacteria from non-recombinant bacteria. Hence, this is not the correct answer.
(4) Recombinant bacteria from non-recombinant bacteria: Blue-white screening is used to select recombinant bacteria (those that have successfully taken up the foreign DNA) from non-recombinant bacteria (those that have not). This is the correct answer.
Conclusion: The correct answer is Option 4: Recombinant bacteria from non-recombinant bacteria.
(1) Vectors from plasmid: Blue-white screening is used to select recombinant bacteria, not vectors from plasmids. Hence, this is not the correct answer.
(2) Gram-positive bacteria from Gram-negative bacteria: Blue-white screening does not differentiate between Gram-positive and Gram-negative bacteria. This is not the correct answer.
(3) Antibiotic-resistant bacteria: While antibiotic resistance may be involved in cloning, blue-white screening is specifically used to distinguish recombinant bacteria from non-recombinant bacteria. Hence, this is not the correct answer.
(4) Recombinant bacteria from non-recombinant bacteria: Blue-white screening is used to select recombinant bacteria (those that have successfully taken up the foreign DNA) from non-recombinant bacteria (those that have not). This is the correct answer.
Conclusion: The correct answer is Option 4: Recombinant bacteria from non-recombinant bacteria.
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