Question:
Blue-white screening is performed to select .......... in vector-based molecular cloning.
Blue-white screening is performed to select .......... in vector-based molecular cloning.
Show Hint
Blue-white screening is a valuable tool in molecular cloning to identify successful transformations by detecting recombinant bacteria.
Updated On: May 6, 2025
- Vectors from plasmid
- Gram-positive bacteria from Gram-negative bacteria
- Antibiotic-resistant bacteria
- Recombinant bacteria from non-recombinant bacteria
Hide Solution
Verified By Collegedunia
The Correct Option is D
Solution and Explanation
Let's analyze each option:
(1) Vectors from plasmid: Blue-white screening is used to select recombinant bacteria, not vectors from plasmids. Hence, this is not the correct answer.
(2) Gram-positive bacteria from Gram-negative bacteria: Blue-white screening does not differentiate between Gram-positive and Gram-negative bacteria. This is not the correct answer.
(3) Antibiotic-resistant bacteria: While antibiotic resistance may be involved in cloning, blue-white screening is specifically used to distinguish recombinant bacteria from non-recombinant bacteria. Hence, this is not the correct answer.
(4) Recombinant bacteria from non-recombinant bacteria: Blue-white screening is used to select recombinant bacteria (those that have successfully taken up the foreign DNA) from non-recombinant bacteria (those that have not). This is the correct answer.
Conclusion: The correct answer is Option 4: Recombinant bacteria from non-recombinant bacteria.
(1) Vectors from plasmid: Blue-white screening is used to select recombinant bacteria, not vectors from plasmids. Hence, this is not the correct answer.
(2) Gram-positive bacteria from Gram-negative bacteria: Blue-white screening does not differentiate between Gram-positive and Gram-negative bacteria. This is not the correct answer.
(3) Antibiotic-resistant bacteria: While antibiotic resistance may be involved in cloning, blue-white screening is specifically used to distinguish recombinant bacteria from non-recombinant bacteria. Hence, this is not the correct answer.
(4) Recombinant bacteria from non-recombinant bacteria: Blue-white screening is used to select recombinant bacteria (those that have successfully taken up the foreign DNA) from non-recombinant bacteria (those that have not). This is the correct answer.
Conclusion: The correct answer is Option 4: Recombinant bacteria from non-recombinant bacteria.
Was this answer helpful?
0
0
Top Questions on cloning
- Which of the following technique is commonly used in animal cloning?
- Match the following column I with column II.
- Plasmids are used in genetic engineering as they are
- This method of finding a gene is used when researchers know very little about the gene they are trying to find. This process results in a complete gene library: a collection of copies of DNA fragments that represent the entire genome of an organism.
- Plants having similar genotypes produced by plant breeding are called:
View More Questions
Questions Asked in AP PGECET exam
The variance for continuous probability function \(f(x) = x^2 e^{-x}\) when \(x \ge 0\) is
- AP PGECET - 2024
- Probability and Statistics
Consider the loop transfer function \(\frac {K(s+6)}{(s+3)(s+5)}\). In the root locus diagram the centroid will be located at:
- AP PGECET - 2024
- Transfer function
When nuclear radiations pass through, gas ionization is produced. This is the principle of which of the following detectors?
- AP PGECET - 2024
- Instrumentation
If \(f = \text{Tan}^{-1}(xy)\) then \((\frac{\partial f}{\partial x})_{(1,2)}\) = _____ .
- AP PGECET - 2024
- Calculus
- Line of Regression of y on x
- AP PGECET - 2024
- Linear regression
View More Questions