- The arrows indicate the sites where the DNA strand is cleaved by EcoRI.
- The cleavage results in two DNA fragments with sticky ends. These sticky ends can anneal to complementary sequences, facilitating the insertion of desired DNA fragments into vectors during recombinant DNA technology.
A battery of emf \( E \) and internal resistance \( r \) is connected to a rheostat. When a current of 2A is drawn from the battery, the potential difference across the rheostat is 5V. The potential difference becomes 4V when a current of 4A is drawn from the battery. Calculate the value of \( E \) and \( r \).