During secondary treatment of sewage, the BOD of the effluent is reduced through the following processes:
- Consumption of organic matter: Aerobic microorganisms in the effluent consume the organic matter.
- Formation of flocs: Microbial activity leads to the formation of flocs, which are aggregations of bacteria and fungi.
- Decomposition of organic matter: Organic matter is broken down into simpler substances like carbon dioxide and water.
- Separation of activated sludge: The flocs settle down as activated sludge, further reducing the organic load.

Amines are usually formed from amides, imides, halides, nitro compounds, etc. They exhibit hydrogen bonding which influences their physical properties. In alkyl amines, a combination of electron releasing, steric and H-bonding factors influence the stability of the substituted ammonium cations in protic polar solvents and thus affect the basic nature of amines. Alkyl amines are found to be stronger bases than ammonia. Amines being basic in nature, react with acids to form salts. Aryldiazonium salts, undergo replacement of the diazonium group with a variety of nucleophiles to produce aryl halides, cyanides, phenols and arenes.