Question

All the statements are true regarding RFLP and RAPD except

• A. RAPD is a quick method compared to RFLP
• B. RFLP is more reliable than RAPD
• C. Species specific primers are required for RAPD
• D. Radioactive probes are not required in RAPD

Hint:

RAPD is a faster method compared to RFLP and doesn't require species-specific primers, making it a useful tool for quick genetic studies.

Answer 1

The Correct Option is C

In RAPD (Random Amplified Polymorphic DNA), random primers are used to amplify DNA regions, so \textit{species-specific primers are not required} in RAPD. This is one of the reasons why RAPD is a quicker and simpler method than RFLP (Restriction Fragment Length Polymorphism).

(1) Why Other Options Are Correct:
- RAPD is a quick method compared to RFLP: RAPD is faster because it uses random primers and does not require restriction enzyme digestion of DNA.
- RFLP is more reliable than RAPD: RFLP is considered more reliable because it involves well-defined markers and precise patterns of DNA fragments.
- Radioactive probes are not required in RAPD: RAPD typically uses non-radioactive methods for detection, making it safer and easier to use than RFLP, which often involves radioactive probes.

Conclusion: The statement species specific primers are required for RAPD is incorrect because RAPD uses random primers.