Question

5\(\prime\)–G\(^\downarrow\)AATTC–3\(\prime\)
3\(\prime\)–CTTAA\(^\uparrow\)G–5\(\prime\)
(a) Name the restriction enzyme that recognises the given specific sequence of bases. What are such sequences of bases referred to as?
(b) What are the arrows in the given figure indicating? Write the result obtained thereafter.

Hint:

Palindromic sequences are unique DNA sequences that read the same in both directions, enabling precise enzymatic cuts.

Answer 1

(a) Restriction enzyme: EcoRI. The sequences are referred to as palindromic sequences. (b) The arrows indicate the site where the restriction enzyme EcoRI cuts the DNA. Result: The DNA is cleaved into fragments with sticky ends, enabling the insertion of foreign DNA during genetic engineering.