List of top Biotechnology Questions asked in IIT JAM BT

The $K_M$ and $v_{\max}$ of an enzyme are 4 mM and 0.1 nM h\(^{-1}\) respectively. In the presence of 1.5 mM inhibitor, the $K'_M$ and $v'_{\max}$ become 6 mM and 0.1 nM h\(^{-1}\). The inhibition constant $K_i$ (correct to 1 decimal place) is ............ mM.

The standard oxidation potentials for oxidation of NADH and H\(_2\)O are +0.315 V and –0.815 V, respectively. The standard free energy for oxidation of 1 mole of NADH by oxygen under standard conditions (correct to 1 decimal place) is ........ kJ. [Faraday Constant is 96500 C mol\(^{-1}\)]

One gram of radioactive nuclei with a half-life of 300 days is kept in an open container. The weight of nuclei remaining after 900 days (correct to 1 decimal place) is .............. mg.

A linear DNA contains five restriction sites for EcoRI and three restriction sites for BamHI. The number of fragments that will be generated after digestion with EcoRI is .............

The population of a bacterial culture increases from one thousand to one billion in five hours. The doubling time of the culture (correct to 1 decimal place) is ............ min.

Antigen and antibody interaction is shown by the following scheme
\[ \text{Ab} + \text{Ag} \; \xrightleftharpoons[k_{-1}]{k_{1}} \; \text{Ab-Ag} \] Where Ab is antibody, Ag is antigen and Ab–Ag is antigen–antibody complex. The values of $k_{1}$ and $k_{-1}$ are $5 \times 10^{5}\ \mu\mathrm{M}^{-1}\mathrm{s}^{-1}$ and $2 \times 10^{-2}\ \mathrm{s}^{-1}$ respectively. The dissociation constant for the complex is .............. nM.

The electric field and capacitance of a capacitor in the absence of dielectric material are $E$ and $C$, respectively. When the capacitor is filled with a dielectric material, the electric field and capacitance become $E'$ and $C'$, respectively. Which of the following is(are) correct?

Kinetic theory of an ideal gas is based upon the following assumption(s)

Bacterial plasmid genes of non-chromosomal origin are associated with

The $\Delta G^\circ$ for homolactic fermentation converting glucose to lactate is –196 kJ mol$^{-1}$. If $\Delta G^\circ$ for the formation of ATP is +30.5 kJ mol$^{-1}$, then

Which of the following is(are) INCORRECT in the regulation of the trp operon?

The cells involved in allergic reactions and containing surface receptors of IgE antibodies and histamine are

A body X of mass $M$ moving with velocity $v$ hits a stationary body Y of mass $m$. If $M \gg m$ and X moves with velocity $v'$ after the collision, then the velocity of Y after an elastic collision is

The position of a particle along the y-axis is $y = P t^4 + Q$. For dimensional consistency, the dimension of $P$ in terms of length [L] and time [T] is

Match the entries in Group I with those in Group II
Group I
P) Nucleolus
Q) Sphaerosomes
R) Peroxisomes
S) Plasmodesmata
Group II
1) lipid storage
2) breakdown of fatty acids
3) transport of macromolecules
4) RNA synthesis

During eukaryotic cell division, the amount of DNA doubles

The nitrogenase of diazotrophs

If an aldol cleavage of glucose-6-phosphate occurs in glycolysis, it will result in

Which of the following is true of protein synthesis ONLY in prokaryotes?

Match the entries in Group I with those in Group II
Group I
P) Phytase
Q) Xylanase
R) Laccase
S) Bromelain
Group II
1) paper and pulp processing
2) delignification
3) gluten complex reduction
4) improve mineral availability
5) phosphorylation

The natural geographical distribution of kangaroos is limited to the Australian continent because

The role of enzyme E synthesized by phage $\phi$X174 during host infection is to

Which of the following is NOT involved in eukaryotic translation?

Which of the following statements is correct?