Question

A linear DNA contains five restriction sites for EcoRI and three restriction sites for BamHI. The number of fragments that will be generated after digestion with EcoRI is .............

Hint:

Linear DNA: fragments = sites + 1. Circular DNA: fragments = sites.

Answer 1

Correct Answer: 6

Step 1: Recall rule for linear DNA fragmentation.
If a linear DNA molecule has $n$ restriction sites, it produces \[ n + 1\ \text{fragments}. \] Step 2: Apply to given case.
EcoRI sites = 5 \[ \Rightarrow 5 + 1 = 6\ \text{fragments}. \] Step 3: Conclusion.
Therefore, EcoRI digestion yields 6 DNA fragments.