List of top Biotechnology Tools and Techniques Questions asked in GATE Biotechnology

For the coupled reactions given below:
Glucose 6-phosphate + H\(_2\)O → Glucose + Pi (Reaction 1)
ATP + Glucose → ADP + Glucose 6-phosphate (Reaction 2) The standard free energy change of ATP hydrolysis at 25 °C is __ kJ/mol.
The equilibrium constants for Reaction 1 and Reaction 2 are 360 and 800, respectively; Gas constant \( R = 8.314 \, {J mol}^{-1} {K}^{-1} \).
(Round off to two decimal places)

An NMR spectrometer operating at proton resonance frequency \( \nu \) of 1 GHz will have a magnetic field strength of __________ Tesla (T).
The gyromagnetic ratio for proton, \( \gamma = 2.675 \times 10^8 \, {T}^{-1} {s}^{-1} \). (Round off to one decimal place)

The percentage of light that would pass through a sample with an absorbance of 2 would be ________%. (Round off to the nearest integer)

A binary mixture of benzene and toluene under vapour-liquid equilibrium at 80 \( ^\circ {C} \) follows ideal Raoult’s law. At this condition, the saturation pressures of benzene and toluene are 101 kPa and 40 kPa, respectively. If the mole fraction of benzene in the liquid phase is 0.6, the corresponding mole fraction of benzene in the vapour phase will be ________. (Round off to two decimal places)

Which of the following methods is/are used for identifying histone modifications?

Correctly match the following Bioinformatic tool/Database with its respective Utility.

Let \( m \) and \( n \) be fixed real numbers. If the function
\[ y(t) = C_1 e^t + C_2 e^{-t} \]
is a solution of
\[ \frac{d^2y}{dt^2} + m \frac{dy}{dt} + n y = 0 \]
for any constants \( C_1 \) and \( C_2 \), then \( m + n \) is equal to

Which of the following compounds have the same degree of reduction per carbon-mole?

Which of the following show(s) optical activity at 100 mM concentration in water?

Which of the following separation processes is/are based on molecular size?

Correctly match the following Product classes with their representative Products.