Question

Match the stationary phase (Column I) with its corresponding chromatography technique (Column II).

• A. P-1, Q-4, R-2, S-3
• B. P-3, Q-1, R-4, S-2
• C. P-3, Q-4, R-2, S-1
• D. P-4, Q-1, R-3, S-2

Hint:

In chromatography, matching the stationary phase with the corresponding technique is crucial for selecting the right method for separation based on the properties of the molecules involved.

Answer 1

The Correct Option is B

The given stationary phases correspond to different chromatography techniques as follows:
- P. Protein A is commonly used in Affinity chromatography (3), where the stationary phase specifically binds to the protein of interest.
- Q. Sephadex is used in Size exclusion chromatography (1), a technique that separates molecules based on their size.
- R. Phenysepharose is used in Hydrophobic interaction chromatography (4), which is based on the hydrophobic interactions between the stationary phase and the analyte.
- S. Diethylaminoethyl cellulose is used in Ion-exchange chromatography (2), a technique where charged particles are separated based on their interaction with the charged stationary phase.
Thus, the correct matching is P-3, Q-1, R-4, S-2, so the correct answer is (B).