Question

Which one makes correct components of a PCR reaction?

• A. DNA template, Primers, DNTPs
• B. DNA polymerase, DNTPs, Primers, DNA template
• C. DNA template, Taq DNA polymerase, Primers, DNTPs
• D. DNA template, DNTPs, Taq DNA polymerase

Hint:

• Essential PCR Components:
  • DNA Template (target DNA)
  • Pair of Primers (forward and reverse)
  • Thermostable DNA Polymerase (e.g., Taq polymerase)
  • Deoxynucleotide Triphosphates (dNTPs: dATP, dGTP, dCTP, dTTP)
  • Buffer (containing Mg\(^{2+}\) ions)
• PCR Cycles: Denaturation \(\rightarrow\) Annealing \(\rightarrow\) Extension.

Answer 1

The Correct Option is C

PCR is a technique used to amplify DNA, and it requires several key components to work effectively:

• DNA template: The sample DNA that contains the region of interest to be amplified.
• Taq DNA polymerase: An enzyme used to synthesize new strands of DNA. It is heat-stable, which is important for the high-temperature steps involved in PCR.
• Primers: Short strands of nucleotides that provide a starting point for DNA synthesis. They are complementary to the sequences at the start of the DNA region of interest.
• dNTPs (Deoxynucleotide triphosphates): The building blocks of DNA that are used by the DNA polymerase to synthesize new DNA strands.

In the given question, the correct combination of components that make up a PCR reaction is: DNA template, Taq DNA polymerase, Primers, dNTPs.