Question

Which of the following is not a step in Multiple Ovulation Embryo Transfer Technology $(MOET)$?

• A. Cow is administered hormone having LH like activity for super ovulation
• B. Cow yields about $6-8$ eggs at a time
• C. Cow is fertilized by artificial insemination
• D. Fertilized eggs are transferred to surrogate mothers at 8 - 32 cell stage

Answer 1

The Correct Option is A

To determine which option is not a step in Multiple Ovulation Embryo Transfer Technology (MOET), we need to understand the standard procedure involved in MOET:

1. Superovulation:

   In this step, the cow is administered with hormones like Follicle Stimulating Hormone (FSH) to induce superovulation. This involves the production of multiple eggs instead of just one. The hormone administered for superovulation typically does not have Luteinizing Hormone (LH) like activity since LH is associated with ovulation and luteinization, not with superovulation itself. Therefore, the statement "Cow is administered hormone having LH like activity for super ovulation" is incorrect in this context.

2. Artificial Insemination:

   After the superovulation, the cow is artificially inseminated to fertilize the eggs. This step is correctly listed in option 3: "Cow is fertilized by artificial insemination."

3. Collection of Embryos:

   Following fertilization, often the cow yields about 6-8 eggs. These eggs are then collected before they are implanted in the surrogate mother. This is represented in option 2: "Cow yields about 6-8 eggs at a time."

4. Transfer to Surrogate Mothers:

   The fertilized eggs, at the stage of 8-32 cells, are transferred to surrogate mothers. This is accurately described in option 4: "Fertilized eggs are transferred to surrogate mothers at 8 - 32 cell stage."

The process of superovulation does not typically involve administering hormones with LH-like activity. Hence, option 1 is not a step in MOET.

Conclusion: The correct answer is "Cow is administered hormone having LH like activity for super ovulation" as it doesn't fit into the standard MOET procedure due to incorrect hormone specification for superovulation.