Question

Which of the following is/are involved in the initiation of DNA replication?

• A. RhoA
• B. oriC
• C. Sigma factor
• D. DnaA

Hint:

Replication starts at {oriC} with the help of {DnaA}, which opens the double helix. Helicase and primase follow to form the replication fork.

Answer 1

The Correct Option is B, D

Step 1: Recall replication initiation in prokaryotes.
DNA replication begins at a specific sequence called the origin of replication (oriC).
Step 2: Role of DnaA.
The DnaA protein binds to oriC and unwinds the DNA, allowing helicase and primase to initiate replication.
Step 3: Eliminate unrelated factors.
- RhoA: Involved in transcription termination.
- Sigma factor: A transcription initiation factor, not replication.
Step 4: Conclusion.
Hence, the correct answer is (B) oriC and (D) DnaA.