Question

Which among the following is true for RFLP?

• A. It cannot be used as a genetic marker
• B. It refers to differences (or variations) in the DNA sequences at sites recognized by restriction enzymes
• C. Restriction enzymes are not used for digesting DNA in this process
• D. It is a PCR based marker

Hint:

• RFLP Restriction Fragment Length Polymorphism.
• Variation in DNA sequence (e.g., SNP) that creates or abolishes a restriction enzyme recognition site.
• Leads to different lengths of DNA fragments after digestion with that restriction enzyme.
• Detected by Southern blotting or PCR-RFLP.
• Used as genetic markers.

Answer 1

The Correct Option is B

RFLP stands for Restriction Fragment Length Polymorphism, which is a process used to analyze variations in DNA sequences. The correct statement regarding RFLP is: It refers to differences (or variations) in the DNA sequences at sites recognized by restriction enzymes.

Here is a brief explanation of why this is correct:

• Genetic Marker Use: RFLP can be used as a genetic marker because the variations detected by RFLP can be linked to specific traits or genetic conditions.
• Role of Restriction Enzymes: Restriction enzymes cut DNA at specific sequences. Differences in these sequences among individuals lead to fragments of varying lengths, which is the basis for RFLP.
• Process Description: In RFLP, DNA is extracted and then digested using restriction enzymes, which cut the DNA at specific recognition sites. This digestion results in fragments of varying lengths depending on the sequence variations present. The fragments are then separated by gel electrophoresis, allowing visualization of the fragment length differences, or polymorphisms.
• Technique Classification: This method is not PCR-based; instead, it relies on the physical cutting of DNA by enzymes and separation by electrophoresis.

Therefore, the correct understanding of RFLP involves recognizing its reliance on restriction enzymes and its ability to highlight DNA sequence variations.