To determine proper pasteurization of milk, the test commonly used is
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Think of the phosphatase enzyme as an indicator. If it's destroyed, the "bad" bacteria were likely destroyed too. A negative phosphatase test means pasteurization was successful.
The Phosphatase test is the standard method used to verify that milk has been properly pasteurized. The enzyme alkaline phosphatase is naturally present in raw milk and is destroyed at the temperature and time combination required for pasteurization. If the test detects the presence of this enzyme, it indicates that the milk was either not heated to the correct temperature or has been contaminated with raw milk after pasteurization.
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