Question

The temperature, time, and pressure combination of autoclaving at laboratory scale is

• A. 101°C, 15 to 20 minutes and 15 psi
• B. 121°C, 15 to 20 minutes and 15 psi
• C. 121°C, 5 to 10 minutes and 10 psi
• D. 120°C, 15 to 20 minutes and 12 psi

Hint:

Autoclaving is an effective method for sterilizing laboratory materials by applying heat and pressure

Answer 1

The Correct Option is B

The common autoclaving conditions used for laboratory-scale sterilization typically involve a temperature of 121°C, with a pressure of 15 psi, and a time duration of 15 to 20 minutes.