Question

The substrate used during DNA replication by the enzyme DNA-dependent DNA polymerase is:

• A. Deoxyribonucleotide triphosphate
• B. Deoxyribonucleoside triphosphate
• C. Ribonucleotide triphosphate
• D. Ribonucleoside triphosphate

Hint:

DNA polymerases always use deoxyribonucleotide triphosphates (not ribose-containing ones) for DNA replication.

Answer 1

The Correct Option is A

DNA-dependent DNA polymerase catalyzes the synthesis of DNA from a DNA template. The enzyme requires deoxyribonucleotide triphosphates (dATP, dGTP, dCTP, dTTP) as substrates. These molecules provide both the building blocks for the new DNA strand and the energy for the polymerization reaction through the hydrolysis of their high-energy phosphate bonds.