Enzymes are biological catalysts. Many enzymes require non-protein components called cofactors for their activity. The protein part of the enzyme, without the cofactor, is called the apoenzyme. When the apoenzyme combines with its cofactor, it becomes a holoenzyme (the complete, active enzyme). Coenzymes are organic cofactors, while prosthetic groups are cofactors tightly bound to the apoenzyme. Cofactors are a broader term encompassing both coenzymes and prosthetic groups. Therefore, only the apoenzyme refers specifically to the protein portion.