Question:

Which of the following factors does NOT affect the rate of an enzyme-catalyzed reaction?

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Enzyme activity is affected by factors like temperature, pH, and substrate concentration, but not by the color of the enzyme. Be sure to focus on factors that impact the enzyme's active site and molecular structure.
Updated On: June 02, 2025
  • Temperature
  • pH
  • Substrate concentration
  • Color of the enzyme
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The Correct Option is D

Solution and Explanation

Enzyme activity is influenced by several factors, but the color of the enzyme is not one of them. Let’s break down the factors that affect enzyme activity:

  • Temperature (Option 1): The rate of enzyme-catalyzed reactions typically increases with temperature, up to a certain point. However, extreme temperatures can denature enzymes and reduce their activity.
  • pH (Option 2): Each enzyme has an optimal pH range in which it functions best. Deviations from this range can affect the enzyme's structure and function, reducing the rate of reaction.
  • Substrate concentration (Option 3): Increasing the substrate concentration typically increases the rate of the reaction, up to a point, as more substrate molecules collide with the enzyme. After a certain concentration, the enzyme becomes saturated, and the rate of reaction levels off.
  • Color of the enzyme (Option 4): The color of the enzyme does not influence its activity. Enzymes function based on their structure and the conditions in which they operate, but the color is not related to their catalytic properties.

Thus, the correct answer is Option (4).

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