Question

The plot of \( 1/V \) versus \( 1/[S] \) where \( V \) is rate of enzymatic reaction and \( [S] \) is substrate concentration is known as:

• A. \( \text{Michaelis-Menton plot} \)
• B. \( \text{Lineweaver-Burk plot} \)
• C. \( \text{Hanes-Woolf plot} \)
• D. \( \text{Eadie-Hofstee plot} \)

Hint:

The Lineweaver-Burk plot assists in identifying enzyme inhibition patterns by analyzing deviations from the linear trend.

Answer 1

The Correct Option is B

The Lineweaver-Burk plot is a double reciprocal graph used to linearize the Michaelis-Menten equation. It plots \( 1/V \) versus \( 1/[S] \), aiding in determining enzyme kinetics parameters such as \( K_m \) and \( V_{\max} \). 
Although useful, it exaggerates errors at low substrate concentrations, leading to preference for nonlinear methods.