Step 1: Understanding the concept.
A tripeptide consists of three amino acids joined in a specific sequence. The order of amino acids matters, and no amino acid can be repeated. Step 2: Counting permutations.
Three different amino acids (ala, gly, val) can be arranged in $3!$ different ways.
\[
3! = 6
\] Step 3: Conclusion.
Thus, six different tripeptides can be formed.