To represent 0.375 in IEEE 754 single-precision format, we first express 0.375 as \( 1.1 \times 2^{-2} \). The sign bit is 0 (since the number is positive), the exponent is \( -2 + 127 = 125 \), which is \( 01111101 \) in binary, and the fraction is \( 10000000000000000000000 \). Therefore, the correct IEEE 754 representation is option (4).