Let the length of the pipe be \( L \), and the speed of sound in air be \( v \).
For the open pipe, the fundamental frequency \( f_1 \) is given by:
\[
f_1 = \frac{v}{2L}
\]
For the closed pipe, the fundamental frequency \( f_1' \) is given by:
\[
f_1' = \frac{v}{4L}
\]
The difference between the fundamental frequencies is given as 100 Hz:
\[
f_1 - f_1' = 100
\]
\[
\frac{v}{2L} - \frac{v}{4L} = 100
\]
\[
\frac{v}{4L} = 100
\]
\[
v = 400L
\]
Now, the second harmonic frequency of the open pipe is:
\[
f_2 = \frac{v}{L}
\]
And the third harmonic frequency of the closed pipe is:
\[
f_3' = \frac{3v}{4L}
\]
The difference between \( f_2 \) and \( f_3' \) is:
\[
f_2 - f_3' = \frac{v}{L} - \frac{3v}{4L} = \frac{v}{4L} = 100 \, \text{Hz}
\]
Thus, the difference is \( 100 \, \text{Hz} \).