Question:
Tetracycline resistance gene of pBR322 consists of this restriction site:
Tetracycline resistance gene of pBR322 consists of this restriction site:
Show Hint
pBR322 is a widely used cloning vector with Ampicillin and Tetracycline resistance genes.
Updated On: Mar 12, 2025
- BamHI
- EcoRI
- PstI
- HindIII
Hide Solution
Verified By Collegedunia
The Correct Option is A
Solution and Explanation
Step 1: Understanding the Restriction Sites in pBR322
- The pBR322 plasmid contains two antibiotic resistance genes:
- Ampicillin resistance (bla gene).
- Tetracycline resistance gene.
- The BamHI restriction site is present in the tetracycline resistance gene, which allows for gene insertion.
- The pBR322 plasmid contains two antibiotic resistance genes:
- Ampicillin resistance (bla gene).
- Tetracycline resistance gene.
- The BamHI restriction site is present in the tetracycline resistance gene, which allows for gene insertion.
Was this answer helpful?
0
0
Top Questions on Plant Biotechnology
Match LIST-I with LIST-II
LIST-I (Scientist) LIST-II (Landmark Discovery) A. Van Overbeek I. Transgenic Bt-cotton B. White II. Introduced coconut water as a media component C. Went III. First synthetic plant tissue culture medium D. Monsanto IV. First plant growth hormone i.e., IAA
Choose the correct answer from the options given below:
- CUET (PG) - 2025
- Plant Biotechnology
- Plant Biotechnology
- Arrange the steps in PEG induced protoplast fusion in correct sequence -
A. Treatment of protoplast mixture with 28-50% PEG for 15-30 minutes.
B. Protoplast aggregation.
C. Washing of protoplast (alkaline medium pH 9-10), and high Ca\textsuperscript{2+ concentration}
D. Selection of protoplasts of different strains / species.
Choose the correct answer from the options given below :- CUET (PG) - 2025
- Plant Biotechnology
- Plant Biotechnology
- Which of the following will be present in the F\textsubscript{1 multicellular embryo, derived from a cross of female plant (A) with male plant B, through "bulbosum method"?
- CUET (PG) - 2025
- Plant Biotechnology
- Plant Biotechnology
Match LIST-I with LIST-II
LIST-I LIST-II A. Gynogenesis I. Callus culture B. Culturing in liquid medium II. Ovary culture C. Androgenesis III. Suspension culture D. Culturing on agar medium IV. Pollen culture
Choose the correct answer from the options given below:
- CUET (PG) - 2025
- Plant Biotechnology
- Plant Biotechnology
Match LIST-I with LIST-II
LIST-I LIST-II A. Protoplast I. Ability of cell to develop into a new plant B. Explant II. Unorganized mass of cells C. Totipotency III. Naked cell D. Callus IV. Any plant tissue used to regenerate new tissue/organ/plant in vitro
Choose the correct answer from the options given below:
- CUET (PG) - 2025
- Plant Biotechnology
- Plant Biotechnology
View More Questions
Questions Asked in TS EAMCET exam
- A body is projected vertically upward with an initial velocity of 40 m/s. Calculate the maximum height reached by the body. (Take \( g = 9.8 \, \text{m/s}^2 \))
- TS EAMCET - 2025
- Kinematics
- The properties required for a material to be used as the core of an electromagnet are:
- TS EAMCET - 2025
- Magnetism and matter
- Which of the following statements are correct?
- TS EAMCET - 2025
- Colligative Properties
- At 27$^\circ$C, 100 mL of 0.05 M Cu$^{2+}$ solution is added to 1 L of 0.1 M KI. Find [KI] in resultant solution.
- TS EAMCET - 2025
- Stoichiometry and Stoichiometric Calculations
- 4.0 g of a mixture containing Na₂CO₃ and NaHCO₃ is heated to 673K. Loss in mass of the mixture is found to be 0.62g. The percentage of sodium carbonate in the mixture is
- TS EAMCET - 2025
- Stoichiometry and Stoichiometric Calculations
View More Questions