To create a recombinant DNA molecule, both the vector DNA and the source DNA need to be cut with the same restriction enzyme. This creates complementary sticky ends that can anneal (base pair) with each other, allowing the source DNA to be inserted into the vector. If different restriction enzymes are used, the sticky ends will not be complementary, and the recombinant DNA molecule cannot be formed.
List-I Transgene | List-II Used for/Products |
---|---|
(A) α-1-antitrypsin | (III) Treat emphysema |
(B) cryIAc | (II) Corn borer |
(C) Antisense RNA | (I) Meloidgyne incognitia |
(D) cryIAb | (IV) Cotton bollworms |
List I | List II | ||
---|---|---|---|
A | Mesozoic Era | I | Lower invertebrates |
B | Proterozoic Era | II | Fish & Amphibia |
C | Cenozoic Era | III | Birds & Reptiles |
D | Paleozoic Era | IV | Mammals |