Question

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

• A. Hind II, Hind II
• B. Hind II, Alu I
• C. Hind II, EcoR I
• D. Hind II, BamHI

Answer 1

The Correct Option is A

To create a recombinant DNA molecule, both the vector DNA and the source DNA need to be cut with the same restriction enzyme. This creates complementary sticky ends that can anneal (base pair) with each other, allowing the source DNA to be inserted into the vector. If different restriction enzymes are used, the sticky ends will not be complementary, and the recombinant DNA molecule cannot be formed.